Robotic Analyzer: SP100 for automated BOD analysis

SP100 BOD

Skalar's SP100 is the most versatile and flexible analyzer of our robotic family. The robust modular concept with two manipulators and moving sample transport mechanism, allows flexible automation for a wide range of applications. The newly designed transport mechanism provides higher sample through-put, the typical bottle capacity of the SP100 for the BOD application is 120 (6 racks of 20 bottles). During the run the worklist can be extended by exchanging sample trays and/or adding additional bottles.

A typical BOD analysis sequence:

Steps of Automation

  • Pippetting of the sample (option)
  • Addition of Nitrification inhibitor (ATU) and/or Seed
  • Addition of dilution water
  • Sample homogenization
  • Measurement of initial Dissolved Oxygen value (DO1)
  • Probe/stirrer rinsing between each measurement
  • Bottle capping/decapping (option)
  • Measurement of final DO2 value
  • Calculation of BOD according required regulations

Samples are pipetted, either manual or automatically (option), into the BOD bottles and the identity and the dilutions are entered or downloaded from LIMS into the sample table . Bottles are placed in the racks and placed on the Robotic Analyzer. Analyzer is started by operator. The manipulator sets the Oxygen probe in position, bottle is capped and filled with Nitrification inhibitor (ATU) and/or seed (option) and dilution water. Sample is then stirred and the initial Oxygen value is measured. The bottle is capped and the Oxygen probe and stirrer are rinsed before going to the next sample. The same procedure is followed until a value is recorded for all samples. The sample racks are placed in a 20°C incubator for 5 days. After incubation the racks are placed on the Robotic analyzer. The operator starts the analyzer. The bottle is capped. The sample is stirred and the final Oxygen value is measured. The BOD value is calculated. This procedure is repeated until all samples are measured. The data can be presented on screen, printed or converted to a file compatible with other software and LIMS's.

SP100 Analyzer features include:

  • Barcode identification (optional)
  • As much as 85% of analyst's time is saved
  • Typical throughput: 200-250 samples/hr (5 probes)